![]() Technical Insightsįollowing a common design standard has been a key strategy for Sc2.0. Not only does this partition the workload associated with chromosome assembly, it also distributes the financial burden as each team is responsible for obtaining their own funding. ![]() While Sc2.0 chromosome design is centralized, involving a close collaboration between yeast geneticists of Jef Boeke’s lab and computational biologists from Joel Bader’s lab at Johns Hopkins University, synthesis and assembly are parallelized between teams around the world. One organizational feature that has driven success of Sc2.0 is the distributive nature of the project. Additionally, because GP-write will involve global participation similar to Sc2.0, it will be important to maintain a collaborative and inclusive culture across international borders. Organizational InsightsĪ well defined plan should be in place at the launch of the project, which all participants must agree upon, including funding, space, personnel, QA/standards, material transfers, publication policy, intellectual property, software, ownership of the project, training and education, and compliance with local laws. To date, 30% of the Sc2.0 genome has been constructed in cells, and the team hopes to have the entire genome – all 16 chromosomes – completed before the end of 2017.Īccording to Mitchell, the genome engineering effort of Sc2.0 can offer some powerful lessons for GP-write, both from an organizational and technical perspective. Earlier this month, the Sc2.0 International Consortium announced the completed design of the Sc2.0 genome, construction of five new synthetic chromosomes, and described the 3D organization of synthetic chromosomes in the nucleus, all of which earned them the March 10 cover story for Science. ![]() The goal of Sc2.0 is to design, build, assemble, and test the function of an entirely synthetic designer yeast genome. In this role, she collaborates with Sc2.0 International Consortium team members spanning 4 continents to provide remote mentorship and solve challenges associated with synthetic chromosome design features and assembly. Mitchell has been leading experimental design and technological development for the Synthetic Yeast Genome Project (Sc2.0) since 2012. If anyone understands the challenges associated with powering a eukaryotic cell with a designer genome built from scratch, it’s Leslie Mitchell, a postdoc in Jef Boeke’s lab at NYU.
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